2QO4
Crystal structure of zebrafish liver bile acid-binding protein complexed with cholic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 |
| Unit cell lengths | 43.355, 43.355, 67.526 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.18757 |
| Rwork | 0.186 |
| R-free | 0.20883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.183 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.083 |
| Number of reflections | 21471 | |
| <I/σ(I)> | 13.8 | 6.3 |
| Completeness [%] | 94.3 | 72.6 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% PEG 4000, 20% isopropanol, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
