2QO2

Human EphA3 kinase and juxtamembrane region, dephosphorylated, apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2006-11-04
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	54.449, 38.359, 76.226
Unit cell angles	90.00, 102.63, 90.00

Refinement procedure

Resolution	19.180 - 1.600
R-factor	0.177
Rwork	0.176
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsf
RMSD bond length	0.008
RMSD bond angle	1.182
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.032	0.024	0.092
Number of reflections	38458
<I/σ(I)>	22.3
Completeness [%]	45.1	48.9	19
Redundancy	1.2	1.3	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 mg/mL Protein, 25% PEG 3350, 0.2M Ammonium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K