2QNR

Human septin 2 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97770
Spacegroup name	C 1 2 1
Unit cell lengths	164.536, 52.295, 110.944
Unit cell angles	90.00, 132.48, 90.00

Refinement procedure

Resolution	20.000 - 2.600
Rwork	0.234
R-free	0.29300
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.388
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	22.998	2.590
High resolution limit [Å]	2.500	5.350	2.500
Rmerge	0.057	0.033	0.435
Number of reflections	22998
<I/σ(I)>	17.1
Completeness [%]	98.9	99.5	95.4
Redundancy	7.2	7.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	PEG 3350, 0.2M Trilithium citrate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, temperature 291K