2QNF
Crystal structure of T4 Endonuclease VII H43N mutant in complex with heteroduplex DNA containing base mismatches
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 64.997, 64.997, 153.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.840 - 3.000 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 12214 | |
| <I/σ(I)> | 20.8 | 5.7 |
| Completeness [%] | 84.6 | 37.6 |
| Redundancy | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 25%(w/v) PEG 3350, 100 mM Bis-Tris, 220mM Li2SO4 and 5% (w/v) glucose, pH 6.5, VAPOR DIFFUSION, temperature 293K, pH 6.50 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Li2SO4 | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | Bis-Tris | ||
| 4 | 1 | 1 | glucose | ||
| 5 | 1 | 2 | glucose | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | Bis-Tris | ||
| 8 | 1 | 2 | glucose |
