2QND
Crystal Structure of the KH1-KH2 domains from human Fragile X Mental Retardation Protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9788, 0.9793, 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.000, 70.700, 68.160 |
| Unit cell angles | 90.00, 107.23, 90.00 |
Refinement procedure
| Resolution | 24.770 - 1.900 |
| R-factor | 0.23003 |
| Rwork | 0.227 |
| R-free | 0.28706 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.183 |
| Number of reflections | 24901 | |
| <I/σ(I)> | 19.6 | 4.8 |
| Completeness [%] | 98.0 | 83.8 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50mM Tris-HCl, pH 8.0, 300mM NaCl, 5mM reduced L-Glutathione, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
