2QN8
Glycogen Phosphorylase b in complex with N-4-nitrobenzoyl-N'-beta-D-glucopyranosyl urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2001-11-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8468 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.612, 128.612, 116.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.181 |
| R-free | 0.19700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 65665 | |
| <I/σ(I)> | 19.6 | 3.1 |
| Completeness [%] | 94.9 | 96.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM Bes, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K |
