2QN7
Glycogen Phosphorylase b in complex with N-4-hydroxybenzoyl-N'-4-beta-D-glucopyranosyl urea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2002-07-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8441 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.335, 128.335, 116.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.830 |
Rwork | 0.204 |
R-free | 0.23000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 78329 | |
<I/σ(I)> | 15.1 | 5.7 |
Completeness [%] | 91.1 | 96.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM Bes, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K |