2QN6
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.119, 51.851, 98.032 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.150 |
| Rwork | 0.217 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A from 2aho |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 31835 | |
| <I/σ(I)> | 7.4 | 2.2 |
| Completeness [%] | 95.3 | 96.6 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 16 % PEG 3350; 0.1 M Magnesium formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
