2QN2

Glycogen Phosphorylase b in complex with Maslinic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	293
Detector technology	CCD
Collection date	2007-02-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.8088
Spacegroup name	P 43 21 2
Unit cell lengths	128.718, 128.718, 116.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.910 - 2.698
R-factor	0.17041
Rwork	0.168
R-free	0.22268
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
RMSD bond length	0.009
RMSD bond angle	1.183
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.910	2.750
High resolution limit [Å]	2.698	2.698
Number of reflections	27542
<I/σ(I)>	17.2	4.2
Completeness [%]	99.8	99.7
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K