2QMY

Quinone Reductase 2 in complex with adrenochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-29
Detector	CUSTOM-MADE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	83.834, 106.461, 56.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.195
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.123
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	17150
<I/σ(I)>	11
Completeness [%]	94.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	300	ammonium sulfate (1.2-2 M), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K