2QMW
The crystal structure of the prephenate dehydratase (PDT) from Staphylococcus aureus subsp. aureus Mu50
Replaces: 2IQ8Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929, 0.97942 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.859, 87.406, 107.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.880 - 2.300 |
R-factor | 0.22734 |
Rwork | 0.225 |
R-free | 0.27610 |
Structure solution method | MAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.114 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.880 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.068 | 0.439 |
Number of reflections | 25930 | |
<I/σ(I)> | 56.5 | 5.5 |
Completeness [%] | 98.4 | 97.8 |
Redundancy | 11.1 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1M Sodium citrate, 3M Sodium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |