2QM8

MeaB, A Bacterial Homolog of MMAA, in the Nucleotide Free Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95
Spacegroup name	P 1 21 1
Unit cell lengths	59.410, 77.040, 79.950
Unit cell angles	90.00, 95.55, 90.00

Refinement procedure

Resolution	29.570 - 1.700
R-factor	0.177
Rwork	0.175
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MeaB Bound to GDP
RMSD bond length	0.011
RMSD bond angle	1.221
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	78116
<I/σ(I)>	23.2	4.7
Completeness [%]	99.8	99.8
Redundancy	6.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M Bis-Tris, pH 5.5, 0.2 M Li2SO4, and 17% PEG 3350., VAPOR DIFFUSION, HANGING DROP, temperature 298K