2QM4
Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9807 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.736, 92.913, 103.693 |
| Unit cell angles | 90.00, 106.22, 90.00 |
Refinement procedure
| Resolution | 37.010 - 2.300 |
| R-factor | 0.18478 |
| Rwork | 0.182 |
| R-free | 0.23891 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.302 |
| Number of reflections | 51723 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 23% PEG 6000, 0.1M Bis-Tris-Propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
