2QLY
Crystral Structure of the N-terminal Subunit of Human Maltase-Glucoamylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9175 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.167, 107.645, 111.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.000 |
R-factor | 0.18075 |
Rwork | 0.178 |
R-free | 0.22382 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 77808 | |
<I/σ(I)> | 17.4 | 3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.2M sodium sulfate, 4% 1,1,1,3,3,3-hexafluoro-2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |