2QLW

Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-23
Detector	MAR CCD 165 mm
Wavelength(s)	0.9079
Spacegroup name	P 32 1 2
Unit cell lengths	69.241, 69.241, 101.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 1.600
R-factor	0.153
Rwork	0.152
R-free	0.18000
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.690
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.097	0.589
Number of reflections	35978
<I/σ(I)>	11.2	3.2
Completeness [%]	97.1	97
Redundancy	5.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2M Sodium Formate, 0.1M Sodium Acetate pH 4.6, 20% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K