2QLT
Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | SBC-3 |
| Wavelength(s) | 1.00932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.636, 55.578, 97.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.930 - 1.600 |
| R-factor | 0.16895 |
| Rwork | 0.168 |
| R-free | 0.19513 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.549 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.930 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.094 | 0.509 |
| Number of reflections | 29314 | |
| <I/σ(I)> | 47.7 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 3.6M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
