2QLS
crystal structure of hemoglobin from dog (Canis familiaris) at 3.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.090, 88.490, 66.370 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 27.870 - 3.500 |
| R-factor | 0.20268 |
| Rwork | 0.199 |
| R-free | 0.29262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.060 |
| RMSD bond angle | 4.126 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.267 | 0.440 |
| Number of reflections | 7539 | |
| <I/σ(I)> | 2.2 | 0.9 |
| Completeness [%] | 97.0 | 94.5 |
| Redundancy | 4.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 45% PEG 6000, TRIS-HCL BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
