2QLM

Glycogen phosphorylase in complex with FN67

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2004-11-19
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8123
Spacegroup name	P 43 21 2
Unit cell lengths	128.633, 128.633, 116.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.18536
Rwork	0.184
R-free	0.21445
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	53619
<I/σ(I)>	21.7	5.8
Completeness [%]	93.1	90
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K