2QLK
Adenovirus AD35 fibre head
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 87.831, 87.831, 53.897 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.400 - 2.020 |
R-factor | 0.216 |
Rwork | 0.200 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h7z |
RMSD bond length | 0.010 |
RMSD bond angle | 1.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.5) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.090 |
High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
Rmerge | 0.082 | 0.043 | 0.501 |
Number of reflections | 9568 | ||
<I/σ(I)> | 9.4 | ||
Completeness [%] | 92.8 | 100 | 58 |
Redundancy | 4.7 | 5.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | Protein in 20mM Tris-HCl, 200mM NaCl, 5mM DTT, reservoir: 30% PEG1000, 0.2M MgBr2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | Protein in 20mM Tris-HCl, 200mM NaCl, 5mM DTT, reservoir: 30% PEG1000, 0.2M MgBr2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |