2QLI
mPlum E16Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.164, 77.904, 96.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.340 |
| R-factor | 0.1834 |
| Rwork | 0.183 |
| R-free | 0.26470 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1g7k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.340 |
| Number of reflections | 95462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 200mM NaCl, 100mM Tris pH8.5, 30% PEG 3400 |
