2QLH
mPlum I65L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.164, 77.419, 96.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7k |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 36504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 200mM NaCl, 100mM Tris pH8.5, 30% PEG 3400 |
