2QLC
The crystal structure of DNA repair protein RadC from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.434, 95.959, 192.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.300 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.27385 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.359 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.122 | 0.699 |
| Number of reflections | 49789 | |
| <I/σ(I)> | 21.68 | 1.81 |
| Completeness [%] | 96.2 | 0.9291 |
| Redundancy | 16 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M Lithium sulfate, 0.1M Tris-HCl, 20% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
