2QL3
Crystal structure of the C-terminal domain of a probable LysR family transcriptional regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 32 |
| Unit cell lengths | 114.277, 114.277, 175.359 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.600 - 2.050 |
| R-factor | 0.17823 |
| Rwork | 0.175 |
| R-free | 0.23853 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.985 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.096 | 0.587 |
| Number of reflections | 159207 | |
| <I/σ(I)> | 16.7 | 1.7 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 1M Sodium-potassium phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
