2QKW
Structural basis for activation of plant immunity by bacterial effector protein AvrPto
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794, 0.9797, 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.465, 94.591, 98.741 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.27364 |
Rwork | 0.271 |
R-free | 0.30337 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.068 | 0.670 |
Number of reflections | 14608 | |
<I/σ(I)> | 20.6 | |
Completeness [%] | 96.2 | 88.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.55M potassium sodium tartrate, 0.1M hepes pH 7.5, 10 mM taurine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.55M potassium sodium tartrate, 0.1M hepes pH 7.5, 10 mM taurine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |