2QKW

Structural basis for activation of plant immunity by bacterial effector protein AvrPto

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-28
Detector	MARRESEARCH
Wavelength(s)	0.9794, 0.9797, 0.9
Spacegroup name	P 21 21 21
Unit cell lengths	75.465, 94.591, 98.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.27364
Rwork	0.271
R-free	0.30337
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.068	0.670
Number of reflections	14608
<I/σ(I)>	20.6
Completeness [%]	96.2	88.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.55M potassium sodium tartrate, 0.1M hepes pH 7.5, 10 mM taurine, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.55M potassium sodium tartrate, 0.1M hepes pH 7.5, 10 mM taurine, VAPOR DIFFUSION, HANGING DROP, temperature 293K