2QKQ
Structure of the SAM Domain of Human Ephrin Type-B Receptor 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 42 |
| Unit cell lengths | 73.566, 73.566, 25.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.790 - 2.100 |
| R-factor | 0.22071 |
| Rwork | 0.220 |
| R-free | 0.24406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f0m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 8210 | |
| <I/σ(I)> | 39 | 2.87 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 2.3 M NaKPO4, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
