2QKO
Crystal structure of transcriptional regulator RHA06399 from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 31 |
| Unit cell lengths | 62.307, 62.307, 164.243 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.430 - 2.350 |
| R-factor | 0.23513 |
| Rwork | 0.233 |
| R-free | 0.27330 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.071 | 0.397 |
| Number of reflections | 29688 | |
| <I/σ(I)> | 41.24 | 4.29 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 289 | 0.1M Bis-tris, 2.5M Ammonium sulfate, 1,5-Diaminopentane di-HCl, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
