2QKN

Crystal structure of Maize cytokinin oxidase/dehydrogenase complexed with phenylurea inhibitor CPPU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	249.544, 50.523, 51.257
Unit cell angles	90.00, 94.01, 90.00

Refinement procedure

Resolution	30.000 - 2.150
Rwork	0.191
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w1o
RMSD bond length	0.062
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.150	2.150
Number of reflections	32292
<I/σ(I)>		3.7
Completeness [%]		86.9
Redundancy		10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15.5% PEG 1500, 0.5% n-octyl beta-D-glucoside, Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K