2QKL
The crystal structure of fission yeast mRNA decapping enzyme Dcp1-Dcp2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Collection date | 2005-10-03 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.723, 49.665, 115.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.330 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.258 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.081 | 0.345 |
| Number of reflections | 11066 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 9.9 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Ammonium Acetate and 5% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
