2QKL

The crystal structure of fission yeast mRNA decapping enzyme Dcp1-Dcp2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Collection date	2005-10-03
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	42.723, 49.665, 115.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.330
R-factor	0.229
Rwork	0.227
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.258
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.390
High resolution limit [Å]	2.330	2.330
Rmerge	0.081	0.345
Number of reflections	11066
<I/σ(I)>	7.6	2.2
Completeness [%]	99.9	99.5
Redundancy	9.9	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Ammonium Acetate and 5% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K