2QK2
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97963, 0.97974, 1.00801 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.370, 113.414, 78.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.580 - 2.100 |
Rwork | 0.202 |
R-free | 0.21700 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.035 | 0.185 |
Number of reflections | 45149 | |
<I/σ(I)> | 17.9 | 4.3 |
Completeness [%] | 98.2 | 91.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.25 M Sodium malonate, Protein concentration 15 mg/ml , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |