2QK1
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97959, 0.97972, 1.01627, 1.12714 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.441, 80.649, 97.919 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.850 - 1.700 |
Rwork | 0.173 |
R-free | 0.19600 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.097 |
Number of reflections | 25288 | |
<I/σ(I)> | 25.4 | 9.4 |
Completeness [%] | 99.0 | 94.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 27% PEG 4000, 100 mM Sodium citrate, 200 mM Ammonium acetate, Protein concentration 15 mg/ml, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |