2QK0
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97964, 1.12713 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.242, 42.533, 51.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.880 - 2.000 |
Rwork | 0.173 |
R-free | 0.23300 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.046 | 0.079 |
Number of reflections | 7855 | |
<I/σ(I)> | 37.6 | 15 |
Completeness [%] | 99.4 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.2 M Sodium malonate, 100 mM Glycine, 1.1 mM Lanthanum chloride, Protein concentration 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.2 M Sodium malonate, 100 mM Glycine, 1.1 mM Lanthanum chloride, Protein concentration 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |