2QK0

Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97964, 1.12713
Spacegroup name	P 21 21 21
Unit cell lengths	27.242, 42.533, 51.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.880 - 2.000
Rwork	0.173
R-free	0.23300
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.079
Number of reflections	7855
<I/σ(I)>	37.6	15
Completeness [%]	99.4	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	1.2 M Sodium malonate, 100 mM Glycine, 1.1 mM Lanthanum chloride, Protein concentration 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	1.2 M Sodium malonate, 100 mM Glycine, 1.1 mM Lanthanum chloride, Protein concentration 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K