2QJX
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97962, 0.97979, 1.03320 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 35.849, 102.502, 31.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.840 - 1.900 |
Rwork | 0.193 |
R-free | 0.22500 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.155 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.034 | 0.068 |
Number of reflections | 17245 | |
<I/σ(I)> | 35.4 | 12.4 |
Completeness [%] | 98.2 | 85.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Tris, 200 mM Lithium sulfate, 29% PEG 4000, Protein stock 15 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |