2QJV
Crystal structure of an iolb-like protein (stm4420) from salmonella typhimurium lt2 at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97913, 0.97883 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 200.140, 200.140, 200.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.514 - 1.900 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.867 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.514 | 29.514 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.080 | 1.900 |
| Rmerge | 0.088 | 0.036 | 0.614 |
| Number of reflections | 104039 | ||
| <I/σ(I)> | 8.27 | 25.1 | 1.7 |
| Completeness [%] | 99.8 | 99.4 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | NANODROP, 0.01M Nickel (II) chloride, 1.0M Lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
