2QJA
Crystal structure analysis of BMP-2 in complex with BMPR-IA variant B12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9796 |
Spacegroup name | P 65 |
Unit cell lengths | 105.740, 105.740, 97.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.580 - 2.600 |
R-factor | 0.21569 |
Rwork | 0.214 |
R-free | 0.25409 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rew |
RMSD bond length | 0.015 |
RMSD bond angle | 1.900 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.610 | 2.650 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.062 | 0.499 |
Number of reflections | 19967 | |
<I/σ(I)> | 10.5 | 2.4 |
Completeness [%] | 98.9 | 90.9 |
Redundancy | 4.22 | 3.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 0.8M K Na phosphate, 25% ethylene glycol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |