2QJ5
PYP ultra-high resolution of a bacterial photoreceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 65 |
| Unit cell lengths | 40.779, 40.779, 117.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.830 - 1.200 |
| R-factor | 0.19975 |
| Rwork | 0.199 |
| R-free | 0.21944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nwz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.045 | 0.240 |
| Number of reflections | 32475 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 71.1 | |
| Redundancy | 3.1 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 39% PEG 3000, 50 mM Tris-HCl pH 9, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
