2QJ1
Crystal structure of infectious bursal disease virus VP1 polymerase incubated with an oligopeptide mimicking the VP3 C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 121.913, 121.913, 359.426 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 3.480 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | VP1 polymease apo form structure (PDB code 2PUS) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.650 |
| High resolution limit [Å] | 3.480 | 3.500 |
| Number of reflections | 20344 | |
| <I/σ(I)> | 10.3 | 2.2 |
| Completeness [%] | 96.4 | 99.3 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10-12% PEG 3350, 0.3-0.5M LiNO3, pH 6.5-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
