2QIU
Structure of Human Arg-Insulin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| 格子定数 [Å] | 80.491, 80.491, 37.640 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 40.260 - 2.000 |
| R因子 | 0.2026 |
| Rwork | 0.197 |
| R-free | 0.24931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zni |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.087 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.101 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.260 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.120 | 0.440 |
| 独立反射数 | 6141 | |
| <I/σ(I)> | 3.4 | 1.2 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 5 | 4.86 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | Sodium Citrate, Acetone, Zinc Sulphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
