2QIP
Crystal structure of a protein of unknown function VPA0982 from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97927 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.207, 75.034, 80.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.620 - 1.480 |
R-factor | 0.16346 |
Rwork | 0.162 |
R-free | 0.18312 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.620 | 1.510 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.046 | 0.391 |
Number of reflections | 31578 | |
<I/σ(I)> | 45.96 | 2.73 |
Completeness [%] | 99.5 | 93.7 |
Redundancy | 5.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 2M Ammonium sulfate, 0.1M Bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |