2QIM
Crystal Structure of Pathogenesis-related Protein LlPR-10.2B from yellow lupine in complex with Cytokinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8030 |
Spacegroup name | P 65 |
Unit cell lengths | 73.822, 73.822, 67.212 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.350 |
R-factor | 0.14843 |
Rwork | 0.147 |
R-free | 0.19438 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | LlPR-10.2F |
RMSD bond length | 0.020 |
RMSD bond angle | 2.043 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.042 | 0.670 |
Number of reflections | 45644 | |
<I/σ(I)> | 56.7 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 1.2 M sodium citrate, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |