2QII

Crystal Structure Of tRNA-Guanine Transglycosylase (TGT) From Zymomonas mobilis Complexed With Archaeosine Precursor, Preq0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2003-03-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	90.622, 65.535, 70.325
Unit cell angles	90.00, 96.47, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.1662
Rwork	0.166
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p0b
RMSD bond length	0.007
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	43081
Completeness [%]	95.6	92.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	PEG 8000, MES, DMSO, DTT, preQ0, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K