2QIH
Crystal structure of 527-665 fragment of UspA1 protein from Moraxella catarrhalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95350 |
| Spacegroup name | P 3 |
| Unit cell lengths | 37.234, 37.234, 224.475 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 223.610 - 1.897 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain A of 1D7M |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.241 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 223.610 | 1.970 |
| High resolution limit [Å] | 1.897 | 1.897 |
| Rmerge | 0.086 | 0.400 |
| Number of reflections | 25857 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 93.5 | 69.9 |
| Redundancy | 9.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 12% PEG 3350, 0.2M Ammonium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
