2QIB
Crystal structure of tetR-family transcriptional regulator from Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.607, 76.292, 65.262 |
Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
Resolution | 62.260 - 1.700 |
R-factor | 0.17921 |
Rwork | 0.177 |
R-free | 0.21412 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.374 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.600 | 1.743 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.094 | 0.624 |
Number of reflections | 52953 | |
<I/σ(I)> | 24.64 | 1.79 |
Completeness [%] | 99.1 | 91.05 |
Redundancy | 9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M KH2PO4, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |