2QI9
ABC-transporter BtuCD in complex with its periplasmic binding protein BtuF
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Collection date | 2007-02-18 |
Wavelength(s) | 0.93209, 0.97934, 0.97980 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.582, 127.396, 97.547 |
Unit cell angles | 90.00, 112.76, 90.00 |
Refinement procedure
Resolution | 29.814 - 2.600 |
Rwork | 0.262 |
R-free | 0.28000 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.814 | 2.690 |
High resolution limit [Å] | 2.586 | 2.600 |
Rmerge | 0.074 | 0.570 |
Number of reflections | 74100 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 30-38% (w/v) polyethylene glycol 400, 50mM either of Tris‑HCl pH 8.4 or Glycine-NaOH pH 9.4 and 400mM (NH4)2SO4., VAPOR DIFFUSION, SITTING DROP, temperature 293K |