2QHW
Crystal structure of a complex of phospholipase A2 with a gramine derivative at 2.2 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 2007-05-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54132 |
Spacegroup name | P 43 |
Unit cell lengths | 53.070, 53.070, 48.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.060 - 2.210 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pyc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.060 | 2.290 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 6606 | |
Completeness [%] | 97.0 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Ammoniumu acetate, PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |