2QHK
Crystal structure of methyl-accepting chemotaxis protein from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 112.268, 112.268, 62.731 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.310 - 1.910 |
R-factor | 0.17369 |
Rwork | 0.172 |
R-free | 0.21274 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.342 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.310 | 1.957 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.133 | 0.620 |
Number of reflections | 15013 | |
<I/σ(I)> | 27 | 5 |
Completeness [%] | 99.3 | 0.934 |
Redundancy | 18.5 | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris, 28% PEG MME 2000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |