2QHB
Crystal structure of NgTRF complexed with telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-06-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.844, 70.844, 68.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.251 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ckx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 15094 | |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 290 | 50mM CaCl2, 28% PEG MME 550, 0.1M Bis-tris pH6.5, EVAPORATION, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CaCl2 | ||
| 2 | 1 | 1 | PEG MME 550 | ||
| 3 | 1 | 1 | Bis-tris | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | CaCl2 | ||
| 6 | 1 | 2 | PEG MME 550 | ||
| 7 | 1 | 2 | Bis-tris | ||
| 8 | 1 | 2 | H2O |
