2QH4

Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	DRX		500
2	Bruker	DRX		600
3	Bruker	AVANCE		800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D NOESY (11echo and watergate)
2	2	1	2D NOESY, 2D TOCSY, Natural abundance 2D 13C HSQC
3	1	2	2D 15N-HMQC, 2D 15N-CPMG-NOESY, 2D JNN-HNN-COSY
4	2	2	2D 13C-HSQC, 2D HCCH-COCSY, 3D HCCH-TOCSY, 3D NOESY-HMQC, 2D 31P spin echo difference HCCH/HSQC/HMQC, 2D CT-CE-HSQC
5	1	3	2D 15N-HMQC
6	2	3	2D 13C-HSQC, 2D filtered-edited NOESYs

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.3	ambient	283
2	6.3	ambient	293

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	20