2QGZ
Crystal structure of a putative primosome component from Streptococcus pyogenes serotype M3. Northeast Structural Genomics target DR58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.169, 76.169, 119.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.050 - 2.400 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.314 |
| Number of reflections | 26035 | |
| <I/σ(I)> | 15 | 11.5 |
| Completeness [%] | 93.0 | 75 |
| Redundancy | 5.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 293 | Ammonium phosphate dibasic, 0.1M Sodium citrate, 20% PEG 1000, MICROBATCH UNDER OIL, temperature 293K |
