2QGY
Crystal structure of an enolase from the environmental genome shotgun sequencing of the Sargasso Sea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.636, 139.168, 121.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.760 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | 0.634 |
| Number of reflections | 69393 | |
| <I/σ(I)> | 13.7 | 3.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 294 | 100 mM MES pH 6.2, 25% PEG 3350, 200 mM Magnesium chloride, VAPOR DIFFUSION, temperature 294K |
