2QGO
Crystal structure of a putative Fe-S biosynthesis protein from Lactobacillus acidophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 |
| Unit cell lengths | 61.261, 61.261, 40.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 2.040 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.050 | 0.213 |
| Number of reflections | 17922 | |
| <I/σ(I)> | 11.4 | 2 |
| Completeness [%] | 96.3 | 73 |
| Redundancy | 6.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 1.8M Ammonium citrate, pH 7.0, temperature 298K |
